The Colorimetric Estimation of the Hydrogen Ion Concentration of Blood.*
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چکیده
With the introduction of Cullen’s calorimetric method (1) of estimating the hydrogen ion concentration of blood serum or plasma, it seemed possible for every laboratoryto have a methodemploying minimum material and apparatus and yet secure reliable results. It is generally conceded that the results obtained by the method on normal human plasma or serum agree very well with the true pH as determined electrometrically. Recently, however, considerable doubt has arisen as to its applicability to abnormal samples. Austin, Stadie, and Robinson (2) observed considerable variation, under pathological conditions in man and in the dog under normal and experimental conditions, in the difference between the calorimetric reading of the diluted serum or plasma at 20” or at 38” and the true pH of the undiluted sample at 38”. They conclude that for use with pathological sera, or under changing experimental conditions, the C or the H correction must be determined on each serum if the calorimetric readings made at 20” or 38” are to be relied upon, and have outlined a procedure for doing this. Hastings, Neill, Morgan, and Binger (3), studying the blood reaction in cases of pneumonia, find it better to use 0.27 as the correction factor instead of 0.22. Cullen, Keeler, and
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تاریخ انتشار 2003